Journal: Renal Failure

Article Title: TNF-α-mediated podocyte injury via the apoptotic death receptor pathway in a mouse model of IgA nephropathy

doi: 10.1080/0886022X.2022.2079527

Figure Lengend Snippet: PCR primer sequence.

Article Snippet: The membranes were blocked with 5% skimmed milk for 2 h, and incubated with specific primary antibodies overnight at 4 °C: Podocin (1:500; cat. no. P0372; Sigma-Aldrich; Merck KGaA), nephrin (1:1,000; cat. no. BAF3159; R&D Systems, Inc.), GAPDH (1:1,000; cat. no. 2118; Cell Signaling Technology, Inc.), TNF-α (1:2,000; cat. no. ab9739; Abcam), caspase-8 (1:1,000; cat. no. ab2590; Abcam), caspase-3 (1:1,000; cat. no. ab184787; Abcam) and TNFR1 (1:1,000; cat. no. sc-8436; Santa Cruz Biotechnology, Inc.).

Techniques: Sequencing

Journal: Renal Failure

Article Title: TNF-α-mediated podocyte injury via the apoptotic death receptor pathway in a mouse model of IgA nephropathy

doi: 10.1080/0886022X.2022.2079527

Figure Lengend Snippet: Proteins and mRNA expressions of podocin and nephrin are significantly reduced in IgAN mouse kidneys. (A) Western blotting results and (B) relative protein expression of nephrin and podocin. (C) Transcription levels of nephrin and podocin ( n = 3). Data are expressed as mean ± SD. * p < 0.05, ** p < 0.01 vs. control group. IgAN, IgA nephropathy.

Article Snippet: The membranes were blocked with 5% skimmed milk for 2 h, and incubated with specific primary antibodies overnight at 4 °C: Podocin (1:500; cat. no. P0372; Sigma-Aldrich; Merck KGaA), nephrin (1:1,000; cat. no. BAF3159; R&D Systems, Inc.), GAPDH (1:1,000; cat. no. 2118; Cell Signaling Technology, Inc.), TNF-α (1:2,000; cat. no. ab9739; Abcam), caspase-8 (1:1,000; cat. no. ab2590; Abcam), caspase-3 (1:1,000; cat. no. ab184787; Abcam) and TNFR1 (1:1,000; cat. no. sc-8436; Santa Cruz Biotechnology, Inc.).

Techniques: Western Blot, Expressing, Control